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pathhunter® express glp1r activated gpcr internalization assay kit  (DiscoverX corporation)

 
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    DiscoverX corporation pathhunter® express glp1r activated gpcr internalization assay kit
    Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) <t>GLP1R</t> internalization assessed in U2OS cells using the <t>PathHunter</t> assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.
    Pathhunter® Express Glp1r Activated Gpcr Internalization Assay Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pathhunter® express glp1r activated gpcr internalization assay kit/product/DiscoverX corporation
    Average 90 stars, based on 1 article reviews
    pathhunter® express glp1r activated gpcr internalization assay kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution"

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics14020463

    Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) GLP1R internalization assessed in U2OS cells using the PathHunter assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.
    Figure Legend Snippet: Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) GLP1R internalization assessed in U2OS cells using the PathHunter assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.

    Techniques Used: RNA Expression, Incubation, Labeling

    eGLP1-ASO-mediated GLP1R internalization in GLP1R-HEK293 cells. Representative confocal images of GLP1R localization in GLP1R-HEK293 cells following incubation with vehicle control ( a ) or 127 I 2 -eGLP1- 34 S 15 -ASO (100 nM) for the time points indicated ( b ). Nuclei were counterstained with DAPI. ( c ) GLP1R mean fluorescence intensity was calculated from a region of interest encompassing the cell border. ( d ) GLP1R-HEK293 cells were incubated with 127 I 2 -eGLP1- 34 S 15 -ASO or exenatide for 5–30 min at indicated concentrations and image analysis performed as in ( b ). For each condition, cells from at least 10 fields of view were analyzed in duplicate wells and data were expressed as mean ± SEM. Scale bar = 10 µm.
    Figure Legend Snippet: eGLP1-ASO-mediated GLP1R internalization in GLP1R-HEK293 cells. Representative confocal images of GLP1R localization in GLP1R-HEK293 cells following incubation with vehicle control ( a ) or 127 I 2 -eGLP1- 34 S 15 -ASO (100 nM) for the time points indicated ( b ). Nuclei were counterstained with DAPI. ( c ) GLP1R mean fluorescence intensity was calculated from a region of interest encompassing the cell border. ( d ) GLP1R-HEK293 cells were incubated with 127 I 2 -eGLP1- 34 S 15 -ASO or exenatide for 5–30 min at indicated concentrations and image analysis performed as in ( b ). For each condition, cells from at least 10 fields of view were analyzed in duplicate wells and data were expressed as mean ± SEM. Scale bar = 10 µm.

    Techniques Used: Incubation, Control, Fluorescence

    Representative confocal images of GLP1R and EEA1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with EEA1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.
    Figure Legend Snippet: Representative confocal images of GLP1R and EEA1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with EEA1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Techniques Used: Incubation, Control

    Representative confocal images of GLP1R and LAMP1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with LAMP1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.
    Figure Legend Snippet: Representative confocal images of GLP1R and LAMP1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with LAMP1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Techniques Used: Incubation, Control

    NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 100 nM or 1 µM 127 I 2 -eGLP1- 34 S 15 -ASO maleimide. ( a ) SIMS images of 100 nM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( b ) SIMS images of 1 mM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( c ) ROI analysis 100 nM incubation GLP1R-HEK293 cells. ( d ) ROI analysis 1mM incubation GLP1R-HEK293 cells.
    Figure Legend Snippet: NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 100 nM or 1 µM 127 I 2 -eGLP1- 34 S 15 -ASO maleimide. ( a ) SIMS images of 100 nM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( b ) SIMS images of 1 mM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( c ) ROI analysis 100 nM incubation GLP1R-HEK293 cells. ( d ) ROI analysis 1mM incubation GLP1R-HEK293 cells.

    Techniques Used: Incubation

    The 127 I 2 -eGLP1- 34 S 19 -ASO click. ( a ) NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 127 I 2 -eGLP1- 34 S 15 -ASO maleimide: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C (eGLP1). ( b ) NanoSIMS images of GLP1R-HEK293 cells incubated with 127 I 2 -eGLP1- 34 S 19 -ASO click: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). White arrows indicate spots 1 and 2 as described in the text. Red boxes indicate spots of 34 S/ 13 C 12 C (ASO), which were observed together with spots of 127 I/ 13 C 12 C (eGLP1). ( c ) ROI analysis of 127 I 2 -eGLP1- 34 S 15 -ASO maleimide-treated GLP1R-HEK293 cells with signal corresponding to spots 1 and 2 indicated by black arrows. ( d ) ROI analysis of 127 I 2 -eGLP1- 34 S 19 -ASO click-treated GLP1R-HEK293 cells.
    Figure Legend Snippet: The 127 I 2 -eGLP1- 34 S 19 -ASO click. ( a ) NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 127 I 2 -eGLP1- 34 S 15 -ASO maleimide: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C (eGLP1). ( b ) NanoSIMS images of GLP1R-HEK293 cells incubated with 127 I 2 -eGLP1- 34 S 19 -ASO click: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). White arrows indicate spots 1 and 2 as described in the text. Red boxes indicate spots of 34 S/ 13 C 12 C (ASO), which were observed together with spots of 127 I/ 13 C 12 C (eGLP1). ( c ) ROI analysis of 127 I 2 -eGLP1- 34 S 15 -ASO maleimide-treated GLP1R-HEK293 cells with signal corresponding to spots 1 and 2 indicated by black arrows. ( d ) ROI analysis of 127 I 2 -eGLP1- 34 S 19 -ASO click-treated GLP1R-HEK293 cells.

    Techniques Used: Incubation



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    pathhunter® express glp1r activated gpcr internalization assay kit  (DiscoverX corporation)
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    DiscoverX corporation pathhunter® express glp1r activated gpcr internalization assay kit
    Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) <t>GLP1R</t> internalization assessed in U2OS cells using the <t>PathHunter</t> assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.
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    https://www.bioz.com/result/pathhunter® express glp1r activated gpcr internalization assay kit/product/DiscoverX corporation
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    pathhunter® express glp1r activated gpcr internalization assay kit - by Bioz Stars, 2026-03
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    pathhunter gpcr internalization assay kit  (DiscoverX corporation)
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    DiscoverX corporation pathhunter gpcr internalization assay kit
    Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) <t>GLP1R</t> internalization assessed in U2OS cells using the <t>PathHunter</t> assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.
    Pathhunter Gpcr Internalization Assay Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pathhunter gpcr internalization assay kit/product/DiscoverX corporation
    Average 90 stars, based on 1 article reviews
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    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) GLP1R internalization assessed in U2OS cells using the PathHunter assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: Verification of 127 I 2 -eGLP1- 34 S 15 -ASO function. ( a ) GLP1R internalization assessed in U2OS cells using the PathHunter assay. ( b ) Malat1 RNA expression in GLP1R-HEK293 cells after incubation with unlabeled or labeled eGLP1-ASO. Data shown as mean ± SEM for 3 independent experiments performed in duplicate.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: RNA Expression, Incubation, Labeling

    eGLP1-ASO-mediated GLP1R internalization in GLP1R-HEK293 cells. Representative confocal images of GLP1R localization in GLP1R-HEK293 cells following incubation with vehicle control ( a ) or 127 I 2 -eGLP1- 34 S 15 -ASO (100 nM) for the time points indicated ( b ). Nuclei were counterstained with DAPI. ( c ) GLP1R mean fluorescence intensity was calculated from a region of interest encompassing the cell border. ( d ) GLP1R-HEK293 cells were incubated with 127 I 2 -eGLP1- 34 S 15 -ASO or exenatide for 5–30 min at indicated concentrations and image analysis performed as in ( b ). For each condition, cells from at least 10 fields of view were analyzed in duplicate wells and data were expressed as mean ± SEM. Scale bar = 10 µm.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: eGLP1-ASO-mediated GLP1R internalization in GLP1R-HEK293 cells. Representative confocal images of GLP1R localization in GLP1R-HEK293 cells following incubation with vehicle control ( a ) or 127 I 2 -eGLP1- 34 S 15 -ASO (100 nM) for the time points indicated ( b ). Nuclei were counterstained with DAPI. ( c ) GLP1R mean fluorescence intensity was calculated from a region of interest encompassing the cell border. ( d ) GLP1R-HEK293 cells were incubated with 127 I 2 -eGLP1- 34 S 15 -ASO or exenatide for 5–30 min at indicated concentrations and image analysis performed as in ( b ). For each condition, cells from at least 10 fields of view were analyzed in duplicate wells and data were expressed as mean ± SEM. Scale bar = 10 µm.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: Incubation, Control, Fluorescence

    Representative confocal images of GLP1R and EEA1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with EEA1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: Representative confocal images of GLP1R and EEA1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with EEA1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: Incubation, Control

    Representative confocal images of GLP1R and LAMP1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with LAMP1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: Representative confocal images of GLP1R and LAMP1 following 30 min incubation with vehicle control ( a ), 100 nM exenatide ( b ), or 100 nM 127 I 2 -eGLP1- 34 S 15 -ASO ( c ). Nuclei were counterstained with DAPI. Nuclei were counterstained with DAPI. Pearson’s correlation co-efficient for GLP1R colocalization with LAMP1 was calculated from cytoplasmic ROIs ( d ). All analyses are presented as mean ± SEM for 3 independent experiments. For each condition, cells from at least 10 fields of view were analyzed from at least 2 technical replicates per experiment. Scale bar = 10 µm.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: Incubation, Control

    NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 100 nM or 1 µM 127 I 2 -eGLP1- 34 S 15 -ASO maleimide. ( a ) SIMS images of 100 nM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( b ) SIMS images of 1 mM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( c ) ROI analysis 100 nM incubation GLP1R-HEK293 cells. ( d ) ROI analysis 1mM incubation GLP1R-HEK293 cells.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 100 nM or 1 µM 127 I 2 -eGLP1- 34 S 15 -ASO maleimide. ( a ) SIMS images of 100 nM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( b ) SIMS images of 1 mM incubation GLP1R-HEK293 cells, 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). ( c ) ROI analysis 100 nM incubation GLP1R-HEK293 cells. ( d ) ROI analysis 1mM incubation GLP1R-HEK293 cells.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: Incubation

    The 127 I 2 -eGLP1- 34 S 19 -ASO click. ( a ) NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 127 I 2 -eGLP1- 34 S 15 -ASO maleimide: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C (eGLP1). ( b ) NanoSIMS images of GLP1R-HEK293 cells incubated with 127 I 2 -eGLP1- 34 S 19 -ASO click: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). White arrows indicate spots 1 and 2 as described in the text. Red boxes indicate spots of 34 S/ 13 C 12 C (ASO), which were observed together with spots of 127 I/ 13 C 12 C (eGLP1). ( c ) ROI analysis of 127 I 2 -eGLP1- 34 S 15 -ASO maleimide-treated GLP1R-HEK293 cells with signal corresponding to spots 1 and 2 indicated by black arrows. ( d ) ROI analysis of 127 I 2 -eGLP1- 34 S 19 -ASO click-treated GLP1R-HEK293 cells.

    Journal: Pharmaceutics

    Article Title: NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution

    doi: 10.3390/pharmaceutics14020463

    Figure Lengend Snippet: The 127 I 2 -eGLP1- 34 S 19 -ASO click. ( a ) NanoSIMS images of GLP1R-HEK293 cells incubated for 30 min with 127 I 2 -eGLP1- 34 S 15 -ASO maleimide: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C (eGLP1). ( b ) NanoSIMS images of GLP1R-HEK293 cells incubated with 127 I 2 -eGLP1- 34 S 19 -ASO click: 12 C 14 N, 34 S/ 13 C 12 C (ASO), 127 I/ 13 C 12 C(GLP1). White arrows indicate spots 1 and 2 as described in the text. Red boxes indicate spots of 34 S/ 13 C 12 C (ASO), which were observed together with spots of 127 I/ 13 C 12 C (eGLP1). ( c ) ROI analysis of 127 I 2 -eGLP1- 34 S 15 -ASO maleimide-treated GLP1R-HEK293 cells with signal corresponding to spots 1 and 2 indicated by black arrows. ( d ) ROI analysis of 127 I 2 -eGLP1- 34 S 19 -ASO click-treated GLP1R-HEK293 cells.

    Article Snippet: GLP1R internalization in U2OS cells was measured with the PathHunter ® eXpress GLP1R Activated GPCR Internalization Assay kit (DiscoverX, #93-0724E3CP0L) using the manufacturer’s protocol as previously described [ ].

    Techniques: Incubation